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CN101475983B 禾谷镰孢菌鉴定及其对多菌灵产生中等抗药性水平确认的一管检测法 失效 - 权利终止

  • Patent Title: 禾谷镰孢菌鉴定及其对多菌灵产生中等抗药性水平确认的一管检测法
  • Patent Title (English): One-tube detection method for indetifying Gibberella zeae and confiming medium pesticide resistance level of Gibberella zeae to carbendazim
  • Application No.: CN200810235087.5
    Application Date: 2008-11-17
  • Publication No.: CN101475983B
    Publication Date: 2011-11-23
  • Inventor: 陈长军 , 周明国 , 王建新
  • Applicant: 南京农业大学
  • Applicant Address: 江苏省南京市卫岗1号南京农业大学科技处钱宝英
  • Assignee: 南京农业大学
  • Current Assignee: 南京农业大学
  • Current Assignee Address: 江苏省南京市卫岗1号南京农业大学科技处钱宝英
  • Main IPC: C12Q1/68
  • IPC: C12Q1/68 ;  C12Q1/04
禾谷镰孢菌鉴定及其对多菌灵产生中等抗药性水平确认的一管检测法
Abstract:
本发明是禾谷镰孢菌(Gibberella zeae，无性态Fusarium graminearum)检测及其对多菌灵产生中等抗药性水平确认的一管检测法，专门用于禾谷镰孢菌菌株及其对多菌灵产生中等抗药性的特异性检测。该法可以从田间采集回来的病穗、病组织和病残体上的病原菌采用ASO-PCR检测，整个过程只需6h，检测准确率达99％。该法具有快速、简便、准确、灵敏的特点。
Abstract(English):

The present invention relates to Gibberella zeae (asexual state Fusarium graminearum) detection and a tube detection method of using the detection to confirm medium level of drug resistance produced by carbendazim, which is special for the specificity detection of Gibberella zeae strain and medium level of drug resistance produced by carbendazim. The method adopts the ASO-PCR detection to the pathogenic germs on the diseased spike, diseased tissue and diseased body collected from field, the entire process only requires 6h, and the detection accuracy rate is 99%. The method has fast, simple, accurate and sensitive features.

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