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发明公开
CN101497928A 鉴定GGⅠ组诺如病毒和GGⅡ组诺如病毒的方法及专用试剂盒 失效 - 权利终止

鉴定GGⅠ组诺如病毒和GGⅡ组诺如病毒的方法及专用试剂盒
摘要：
本发明公开了一种鉴定GGI组诺如病毒和GII类诺如病毒的方法及其专用试剂盒。该试剂盒包括链霉亲和素包被的磁珠和鉴定GGI组诺如病毒和GTT类诺如病毒的基因芯片；该基因芯片，包括芯片基片以及固定与其上的检测GGI组诺如病毒的探针和检测GG II组诺如病毒的探针；检测GGI组诺如病毒基因片段的探针为序列1所示的片段；检测GG II组诺如病毒基因片段的探针为序列2所示的DNA片段和序列3所示的DNA片段。该方法是待测样品RNA反转录后用生物素标记，然后用上述试剂盒检测。本发明的试剂盒可筛查诺如病毒，并将其分类直接鉴定为GGI组诺如病毒或GG II组诺如病毒，真阳性率达到100％，真阴性率达到100％，鉴别GGI组诺如病毒或GG II组诺如病毒的准确率达到100％。
摘要（英）：

The invention discloses a method for identifying GGI group norovirus and GII group norovirus and a special reagent kit. The reagent kit comprises streptavidin-coated magnetic beads and a gene chip for identifying the GGI group norovirus and GII group norovirus; the gene chip comprises a chip substrate and probes for detecting the GGI group norovirus and GG II group norovirus and are fixed on the chip substrate; the probe for detecting the gene segment of the GGI group norovirus is a segment shown in a sequence 1; and the probe for detecting the gene segment of the GG II group norovirus is a DNA segment shown in a sequence 2 and a DNA segment shown in a sequence 3. The method comprises the following steps: RNA of the sample to be detected is biotin-labeled after reverse transcription and then is detected with the reagent kit. The reagent kit can screen noroviruses and classify the noroviruses and directly identify the noroviruses as the GGI group norovirus or the GG II group norovirus, achieves the true positive rate and the true negative rate of both 100 percent and the accuracy rate of identifying the GGI group norovirus and GII group norovirus of 100 percent.

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