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发明公开
CN101502673A 一种可注射壳聚糖/甘油磷酸钠/胶原水凝胶的制备方法 无效 - 撤回

一种可注射壳聚糖/甘油磷酸钠/胶原水凝胶的制备方法
摘要：
本发明公开了一种可注射壳聚糖/甘油磷酸钠/胶原水凝胶的制备方法，属于生物技术与组织工程领域，特别涉及一种温敏型可注射材料制备的技术。其特征是：用2.2％(W/V)的壳聚糖溶液与50％(W/W)的β－甘油磷酸钠溶液低温下混合制成壳聚糖/甘油磷酸钠溶液，接着将该体系与胶原溶液按1∶10－10∶1的体积比低温下混合，在室温(18℃)下保持液态，在体温下12分钟转变为凝胶。本发明的有益效果是，壳聚糖/甘油磷酸钠/胶原水凝胶能够在pH值为生理范围内(pH值为7.0～7.2)时低温保持液态，温度升高到体温(37℃)时转变为凝胶。由于加入了理想的细胞生长基质胶原，使得此种水凝胶具有良好的生物相容性。
摘要（英）：

The invention discloses a method for preparing injectable chitosan/sodium glycero-phosphate/collagen hydrogel, belonging to the field of biotechnology and tissue engineering; the invention particularly relates to a technology for preparing temperature sensitive type injectable material. The method is characterized in that 2.2% (W/V) of chitosan solution is mixed with 50% (W/V) of beta-sodium glycero-phosphate solution at low temperature to prepare chitosan/sodium glycero-phosphate solution; then, the solution is mixed with collagen solution following a volume ratio of 1:10-10:1 at low temperature; the mixture is retained in the liquid state at room temperature( 8 DEG C) and is turned into gel at body temperature within 12min. The invention has the beneficial effects that the chitosan/sodium glycero-phosphate/ collagen hydrogel can be maintained in the liquid state at low temperature when pH value is within physiological range (pH value ranges from 7.0 to 7.2); the mixture is turned into gel when the temperature is raised to body temperature (37 DEG C). As ideal cell growth substrate collagen is added, the hydrogel features fine biocompatibility.

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