The invention relates to a bactericidal composition, the active ingredients of which are Zhongshengmycin and calcium copper sulfate; the mass proportion of the Zhongshengmycin and the calcium copper sulfate is 10:1-1:50, and the better proportion thereof is 5:1-1:20. After the Zhongshengmycin and the calcium copper sulfate are compounded, the bactericidal composition has obvious synergistic action and is used for the control of diseases in crops such as fruit trees, cotton, rice, vegetables and the like, especially bacterial diseases.