The invention relates to a culture method of biological cleaning strains in low-concentration volatile mixed organic waste gas. The method comprises the following steps: a), culturing a first strain; b), carrying out the combined inoculation of the first strain and a second strain and using tap water to prepare the sludge concentration of the mixed strains; and c), using mixed organic matter in the waste gas as a substrate to be cast into an insition culture medium so as to culture and train insitions. The first strain is prepared by culturing and training from activated mud taken from a printing and dyeing sewage treatment plant and is a mixed strain of flavobacterium and Bacillus cereus, and the second strain is prepared by collecting activated mud collected from a secondary sedimentation tank of a petrochemical sewage treatment plant and is a mixed strain comprising the flavobacterium, the Bacillus cereus, xylose oxidase Achromobacter and Pseudomonas stutzeri. The method facilitates the balanced growth of various cultured microorganisms and can simultaneously decompound various target configuration compounds at high efficiency.