The invention relates to a finishing method of biological enzyme in wood fiber fabrics, which is characterized in that a nonionic wetting agent is adopted for preliminary treatment, cellulase and pectase are added for enzyme treatment, and finally, hot water is used for inactivation and washing. By adopting the method, the fabrics containing wood fibers have smooth and fluffy hand feelings, the service performance thereof is greatly improved, and the water absorption performance of the fabrics is further improved; since the cellulase and the pectase are used as finishing agents, chemical pollution can not be caused in production, hazardous substances can not remain in the fabrics, and the cellulase and the pectase can degrade after being discharged in an environment and can not pollute the environment, thereby the finishing method belongs to an environment friendly afterfinish technology. Through detection, after the treatment by the method, the water absorption performance of the wood fiber fabrics is improved by more than 34%, the strength damage is within 10%, the drapability, the white content and the pliability are obviously improved, and the improved magnitude is more than 30%.