The invention relates to a method for detecting the content of trace elements in cheese, which comprises the following steps of: weighing a cheese sample and drying the sample for completely evaporating the water in the sample, then weighing the sample after drying and utilizing the weight difference of the sample before and after the drying to calculate the water content in the sample, and then taking the dried sample for being grinded and homogenized, adding hydrochloric acid with the volume ratio of 1:1 for dissolving the dried sample, and adding 2% of agar solution as a suspension stabilizer for even mixing and stable dissolving, thereby obtaining solution to be detected; and utilizing inductive coupling plasma emission spectroscopy or atomic absorption spectrometry for detecting the content of trace elements in the solution to be detected. The detection method is simple, rapid and accurate, and has high-sensitivity.