A manufacturing method of bio-bacterial manure comprises the steps of a) based on weight percentage of the main materials of the fertilizer, adding 1-25% of 60-80-mesh straw fiber powder, 3-20% of 80-200-mesh porous laminated silicate powder and 3-50% of harmless livestock excretion which is biologically degraded in the main material of fertilizer, drying and shaping, (b) after drying and shaping the main materials of the fertilizer, obtaining particle fertilizer whose water content is less than 3%, spraying bio-bacterial and nutrition liquid on the particle fertilizer through the way of spraying such that the mixture is quickly absorbed by the particle fertilizer; (c) at last, putting the particle fertilizer absorbing the bacterial solution in a storage chamber or container designed according to bio-fermenting bacterial, carrying out and finishing bio-fermenting process during storage period under the condition of controlling temperature, moisture and pH value, at last, obtaining qualified bio-bacterial manure product. The invention drastically changes the production technical process of the bio-bacterial manure and has low energy consumption; the quality of the bio-bacterial manure is good.