The invention relates to a spray drying method of enzyme preparation zymotic fluid. A windshield disk is arranged on the medial axis of a cyclone separating device and a material collector and in the material collector, and the windshield disk is 2-10 cm away from a feeding hole and just opposite to a discharging hole below the cyclone separating device. Compared with the prior art, the invention has high wind inlet temperature, high production efficiency of equipment, short contact time of 30s for heat-sensitive material and hot wind and high yield of enzyme activity of more than 75 percent, improves the production efficiency by more than 20 percent, especially has obvious effect for spray drying of high-temperature-intolerant prolease preparation zymotic fluid and feeding enzyme preparation zymotic fluid with low cost and is widely suitable for spray drying of heat-sensitive materials such as other enzyme preparation zymotic fluid, extraction liquid, zymogen liquid, and the like.