The invention relates to a kit, and aims at providing a kit used for detecting candida paropsilosis in the intestinal tract by a fluorescence quantitative PCR method. The kit comprises a standard positive template and two primers of a specificity amplification candida paropsilosis ITS zone used in the PCR amplification process, wherein the gene of a coded amplification product has the nucleotide sequence shown by SEQ ID NO: 3. The operation is simple and convenient and fast; the specificity is good, and the sensitivity is high; the detection based on nucleotide is not limited by culture conditions; the quantitative detection can truly reflect the situations of candida paropsilosis permanent planting and infection in the intestinal tract; and high-flux sample detection can be implemented simultaneously. The kit can carry out fast and quantitative detection to the candida paropsilosis in the intestinal tract, can replace the traditional diagnostic method of isolated culture which is usedfor a long time, and is suitable for being widely popularized and applied in clinical laboratory.