The invention discloses a method for breeding large Sepiella maindroni ink sacs, which comprises the steps of temporary parent culture, seedling culture, larva culture and adult culture, wherein an amino acid composition of parent pallium is added to sieve temporarily cultured Sepiella maindroni so as to select parent Sepiella maindroni with quick ink sac hyperplastic capability in the step of temporary parent culture in the breeding process; artemia or remiped which is reinforced by amino acid related to ink sac hyperplasia is used to breed Sepiella maindroni larva in the larva culture step;and auxiliary bait containing the amino acid related to ink sac hyperplasia is used to breed Sepiella maindroni adults in the adult culture step. The method has the characteristics of sieving cuttlefish with ink sac hyperplastic capability and improving the growth speed and the medical value of the Sepiella maindroni ink sacs, has simple steps and obvious effects, and is suitable for large-scale promotion.