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发明授权
CN101560515B 一种小麦矮腥黑粉菌的特异基因序列、特异性SCAR标记及PCR检测方法 失效 - 权利终止

一种小麦矮腥黑粉菌的特异基因序列、特异性SCAR标记及PCR检测方法
摘要：
本发明涉及“一种小麦矮腥黑粉菌特异基因序列、特异性SCAR标记及其应用”，小麦矮腥黑粉菌特异性基因序列，如Seq No.1，Seq No.2，Seq No.3所示，根据这些特异性片段的核苷酸序列获得的高特异性和高灵敏度的SCAR标记如Seq No.4，Seq No.5或Seq No.6所示的核苷酸序列，可为PCR技术检测小麦矮腥黑穗病提供多种引物选择。根据SCAR标记序列设计的高特异性引物TCKSF(1-2)/TCKSR(1-2)的检测灵敏度可达到1ng/25μl，可将小麦矮腥黒粉菌从众多相似菌株鉴别出来。
摘要（英）：

The invention relates to a specific gene sequence of tilletia controversa kuhn, a specific SCAR marker and application thereof. For the specific gene sequence of the tilletia controversa kuhn, according to the nucleotide sequences of the specific fragments shown in Seq No.1, Seq No.2 and Seq No.3, the nucleotide sequences of the SCAR marker, which are shown in Seq No.4, Seq No.5 or Seq No.6 and are high in specificity and sensitivity, can be obtained, so that various primer selections are provided for the PCR technique to detect the wheat dwarf bunt. The detection sensitivity of the high-specificity primer TCKSF(1-2)/TCKSR(1-2) which is designed according to the sequences of the SCAR marker reaches 1ng/25mu l, so that the tilletia controversa kuhn can be identified from various similar strains.

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