The invention relates to a traditional Chinese medicine injection for treating canine parvovirus disease, wherein crude drugs of the injection consist of following components: 100 parts by weight (pbw) to 500pbw of Chinese pulsatilla root, 100pbw to 300pbw of indigowoad leaves, 100pbw to 400pbw of honeysuckle flowers, 10pbw to 100pbw of rhubarb, 100pbw to 200pbw of flos sophorae, 100pbw to 200pbwof rhizoma coptidis, 100pbw to 300pbw of radix astragali, 100pbw to 300pbw of dark plum fruits, 100pbw to 200pbw of radix curcumae, 100pbw to 300pbw of myrobalam, 100pbw to 300pbw of hairyvein agrimonia herb and bud and 100pbw to 200pbw of rhizoma pinelliae processed with gingers; the invention also discloses a preparation method of the injection, which comprises the steps of: conducting steam distillation extraction on the Chinese pulsatilla root and the radix curcumae, conducting alcohol extraction on the hairyvein agrimonia herb and the rhizoma pinelliae, and conducting water-alcohol extraction on the indigowoad leaves, the honeysuckle flowers, the rhubarb, the flos sophorae, the rhizoma coptidis, the radix astragali, the dark plum fruits and the myrobalam, thereby obtaining the injection. The injection can achieve the purpose of treating the canine parvovirus disease by clearing away heat and toxic material, dispelling heat and relaxing bowels, relieving diarrhea with astringents,cooling blood to stop blood, calming the adverse-rising energy and arresting vomiting. The injection is the traditional Chinese medicine injection prepared by extracting and refining traditional Chinese medicinal materials, thus having no toxicity or side effect on the canine, with quick effect and high cure rate after injection.