The invention relates to a method for preparing molecularly imprinted polymers of ethinylestradiol analogue. The method comprises the following preparation steps: a, taking 17-ethenyl estradiol as a template, mixing template molecules, functional monomers, a crosslinking agent, a pore-foaming agent and an initiator, performing ultrasonic polymerization on the mixture for 12 to 24 hours at the temperature of 60 DEG C in the atmosphere of nitrogen, and obtaining polymers; b, performing centrifugation on the prepared polymers, discarding supernatant, using a methanol-acetic acid mixture for soakcleaning and removal of the template molecules, and obtaining the molecularly imprinted polymers; and c, performing vacuum drying on the polymers. Because the template is easy to separate from ethinylestradiol and estradiol on a chromatographic column, the prepared MIP cannot influence the accuracy of a result due to the leakage of the template when being used for solid-phase extractive materials.Moreover, the prepared MIP reveals obvious compatibility to the 17-ethenyl estradiol, the ethinylestradiol and the estradiol, and corresponding non-imprinted polymers (polymers prepared without the template in the same condition) reveal low compatibility.