The invention provides an elisa kit for detecting cephalo-type medicine, containing: an ELIAS plate peridium of which is provided with peridium source, an enzyme label, cephalo-type medicine specific antibody working solution (contained when the peridium on the ELIAS plate is antigen and the enzyme label is enzyme label anti antibody or when the peridium on the ELIAS plate is anti antibody and theenzyme label is enzyme label antigen), ceftiofur standard solution, substrate developer, stop solution, concentrated cleaning solution and concentrated complex solution. The invention also disclosesa method applying the elisa kit for detecting cephalo-type medicine, including: firstly sample preprocessing is carried out, then the elisa kit is used for detection, and finally the detection result is analyzed. The elisa kit provided by the invention can be applied to detecting residual quantity of cephalo-type medicine in samples such as animal tissue (chicken, pork, fish and shrimp) and milk, etc, the operation is easy, the cost is low, the sensitivity is high, and the elisa kit is applied to on-site supervision and numerous sample screening.