The invention provides a backlight short circuit protection circuit which comprises two backlight power supply circuits, a pulse-width modulation integrated circuit, a first input circuit and a secondinput circuit. Each backlight power supply circuit comprises two backlight lighting tubes and two detection output ends. The pulse-width modulation integrated circuit comprises a current sampling end, and when the voltage of the current sampling end is less than a certain voltage value, the pulse-width modulation integrated circuit stops working. The first input circuit comprises a first transistor and a second transistor, wherein a control pole of the first transistor receives signals of a detection output end, a first conduction pole of the second transistor is connected to the current sampling end and receives the signals of the another detection output end, and a second conduction pole of the second transistor is grounded. The second input circuit receives the signals of the other twodetection output ends, and controls the opening and closure of the second transistor by the first transistor according to the signals. The backlight short circuit protection circuit is safe and has high reliability.