The invention relates to a micro-ecological preparation and the application thereof, in particular to a micro-ecological preparation containing a variety of probiotics and the application thereof. Themicro-ecological preparation contains any three or four of the components including CGMCC No. 2383 bacillus licheniformis powder, bacillus subtilis powder, CGMCC No. 2386 enterococcus faecalis powder, lactobacillus acidophilus powder, and CGMCC No. 2388 saccharomyces cerevisiae. The micro-ecological preparation has content of live bacteria, has the adversity resistance such as gastric acid resistance, bile salt resistance, high-temperature resistance, common antibiotic resistance, and the like and the probiotic functions of producing acid and enzyme and resisting pathogenic bacteria. The variety and the proportion of the probiotics and carrier can be determined according to different kinds of animals and different animal growth phase. The micro-ecological preparation can improve the feedutilization efficiency, increase the yield of meat, eggs and milk, promote the growth of the animal, improve the immunity and the disease resistance of the animal, replace the antibiotic and improve the quality of the animal product.