The inventioin discloses a delinting method of cotton seed by using acid solution, particularly a delinting method of cotton seed by using acid solution, at least comprises following steps: adding acid and mixing, dehydrating, drying, friction, selecting, the improvements of the adding acid and mixing, and steps are as follows: rough cotton seeds are disposed in a revolving screen with a feed portand a discharge port, inner wall of the revolving screen is provided with a propeller blade, and lower of the revolving screen is immersed into acid solution in the acid solition pool, the rough cotton seeds are transported from feed port to discharge port by rotatioin of the revolving screen and pushing of the propeller blade, adding and mixing of the cotton seeds is completed. Comparing with the prior art, the processing method according to the invention has advantages of lower damage to seeds, lower waste, and good quality during processing.