Some methods and related apparatus for manipulating a fluid conduit for insertion into a substantially re-sealable membrane include determining an orientation and position of a fluid conduit relative to the membrane. In an illustrative example, a syringe needle having a beveled leading edge may be manipulated by an automated device to be oriented and aligned with an aperture made upon a previous insertion of a needle into a membrane. In some examples, a predetermined number of insertions may be made in the same aperture by aligning and orienting one or more needles with the aperture. In some examples, multiple needle insertions may be controlled to produce apertures that are substantially spaced apart. Such procedures may, for example, advantageously extend the integrity of the membrane against leakage and/or contamination.