The invention provides a training method for odor discrimination based on the flapping reflex of bees to an electric shock. The method comprises the following steps: 1) capturing bees, placing the bees in ice for anesthetization for1 to 2 minutes, and then transferring the bees to tailor-made electric cages by 20 to 40 bees per cage; accommodating for 1 to 3 hours, placing the tailor-made electric cages loaded with the bees in a gas-passage chamber and starting training, wherein, the gas supply lasts 10 seconds, and the electric shock is given 5 seconds after the gas supply and lasts 10 seconds for each training, and conducting the training for the next time after an interval of 20 to 30 minutes, and training each cage of bees for 3 to 5 times; and 3) recording the flapping reflex of the bees upon the gas supply which lasts 10 seconds without electric shock in the detection. The training method of the invention can effectively train the bees to establish the flapping reflex, particularly, the trained bees have the flapping reflex when smelling characteristic odor, in this way, the invention can provide assistance in various operations involving national defense and civil security,such as characteristic odor detection, drug and explosive enforcement, exit-entry security inspection and the like.