The invention discloses a high-efficiency extraction method of effective ingredients in golden camellia tea and the application thereof in cigarette preparation. The extraction method comprises the steps of crushing, enzymolysis, alcohol extraction, separation and concentration, wherein the enzymolysis is carried out by using cellulose and prolease. The application of the extraction method in cigarette preparation comprises the following steps: adding extracted effective-ingredient concentrated solution of golden camellia tea into a cigarette group according to a mass ratio of 0.1 per thousand to 10 per thousand to prepare cigarettes. By adopting the extraction method, the effective ingredients in the golden camellia tea are extracted with high efficiency and low cost. The extracted effective-ingredient concentrated solution of the golden camellia tea is applied to the cigarette preparation and changes smoke gas to be soft, delicate and clear, the irritation is reduced, residues reduced in the oral cavity are reduced, the sweet backing sense is obviously strengthened, and the cigarette quality is obviously improved.