The invention relates to a subtype diagnostic kit of animal chlamydia disease and a method thereof. In the invention, a pair of aiming upstream and downstream polymerase chain reaction oligonucleotide primers according to the three animal chlamydia ompA gene sequences of animal chlamydias, one specific fragment of all the animal chlamydias can be expanded, and the effective detection of all the chlamydias of animal bedsonia is realized. On the basis of the primers, three sections of fragments with different lengths and sizes can be synchronously expanded by a single pipe in a clinical nucleicacid specimen and can be clearly resolved in common electrophoresis so as to realize the synchronous detection of three animal chlamydias. The invention utilizes a sleeve type multiple PCR method to synchronously detect the three chlamydias of pig chlamydia, psittacosis chlamydia and pulmonitis chlamydia by the single pipe, thereby lowering the cost and the workload of the animal chlamydia disease diagnosis of an animal chlamydia laboratory. The invention has strong specificity, high sensitivity, high repeatability, simple and convenient operation and no complicated post-treatment.