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发明公开
CN101629202A 一种提高肉苁蓉培养细胞苯乙醇糖甙含量的方法 无效 - 撤回

一种提高肉苁蓉培养细胞苯乙醇糖甙含量的方法
摘要：
本发明涉及一种提高肉苁蓉培养细胞苯乙醇糖甙含量的方法。其操作步骤主要包括：1.肉苁蓉细胞的继代培养；2.肉苁蓉细胞的液体培养和超声波处理过程；3.苯乙醇糖甙含量测定过程。利用超声波对悬浮培养的肉苁蓉细胞进行刺激，是提高细胞苯乙醇糖甙含量的有效手段。本发明操作简便，效果良好，扩展性强，可应用于肉苁蓉细胞生物反应器大规模培养过程，提高培养细胞药用次生代谢产物的含量。
摘要（英）：

The invention relates to a method for improving the content of phenethyl alcohol glucoside in cistanche salsa cultured cells, which mainly comprises the following operation steps: carrying out the successive transfer culture of cistanche salsa cells; 2. carrying out the liquid culture and ultrasonic treatment of the cistanche salsa cells; and 3. measuring the content of the phenethyl alcohol glucoside. Using ultrasonic waves to stimulate the cistanche salsa cells in suspension culture is an effective means for improving the content of phenethyl alcohol glucoside in the cells. The method has simple operation, good effect and strong expansibility, can be applied to the mass culture process of a cistanche salsa cell bioreactor, and can improve the content of medicinal secondary metabolites of the cultured cells.

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