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发明公开
CN101629214A 手足口病相关病毒平行检测液相芯片及其制备方法与应用 无效 - 驳回

手足口病相关病毒平行检测液相芯片及其制备方法与应用
摘要：
本发明公开了一种手足口病相关病毒平行检测液相芯片及其制备方法与应用。本发明的手足口病相关病毒平行检测液相芯片，主要由微球，特异性探针，生物素化的通用引物，链霉亲和素-藻红蛋白组成，其特征是，所述微球是26、36和46号微球；所述特异性探针是针对EV71、CoxA16和Echo9病毒VP1区域的氨基化的特异性探针，通用引物是经活化的生物素标记的肠道病毒通用引物。本发明不同的探针可以和不同的目的分子进行结合，反应后通过激光检测球形基质的色彩编号可以对不同的检测反应加以区分，既具有定性或定量的特点，又具有高通量的优点，因而可更加省时省力，同时节约了检测成本。
摘要（英）：

The invention discloses a hand-foot-and-mouth disease (HFMD) related virus parallel detection liquid-phase chip and a preparation method and an application thereof. The HFMD related virus parallel detection liquid-phase chip in the invention mainly comprises microballoons, specific probes, biotinylated universal primer and streptavidin-phycoerythrin, which is characterized in that microballoons in the chip are Number 26, Number 36 and Number 46 microballoons; the specific probes are aminated specific probes aiming at virus VP1 area of EV71, CoxA16 and Echo9; the universal primer is enterovirus universal primer marked by activated biotin. In the invention, different probes can combine with different target molecules, different detection reactions can be classified by using laser for detecting color numbers of sphere matrix after reaction, thus having the quantitative or qualitative characteristics and high throughput advantage, thereby saving time and power and detection cost greatly.

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