The invention relates to a method for improving TDS protocol analysis accuracy in a packet-losing environment. The method comprises the following steps: after receiving application data finishing TCP message recombination, a TDS protocol analysis module recognizes the generation of the packet-losing condition by extracting a TDS message header in the application data and analyzing the validity ofthe TDS message header; after the packet-losing condition is generated and recognized, whether following generated application data are subject to the previous transport service data unit (TDU) is judged by the characteristics of TDS half-duplex communication, thereby ensuring that the application data subject to the TDU are accurately and completely received and then concrete protocol analysis is carried out on the application data under the condition of unavailable correct assembly according to a TDS protocol when the application data in the TDU lose, and improving the accuracy of TDS protocol analysis.