The invention relates to a sterilization combination containing benzene kresoxim-methyl ((aE)-2-[(2,5-dimethylphenoxy)methyl]-alpha-(methoxymethylene)-Benzeneacetic acid methyl ester) and difenoconazole (1-((2-(2-chloro-4-(4-chlorophenoxy)phenyl)-4-methyl-1,3-dioxolan-2-yl)methyl)-1H-1,2,4-triazole) with the weight ratio being 1:80-80:1 or preferably 1:10-10:1. The sterilization combination can be prepared into water dispersible granules (WDG), wettable powder (WP), suspension concentrate (SC), emulsion in water (EW), micro-emulsion (ME) and emulsion concentrate (EC). The invention has the advantages of reasonable components, good sterilization effect and low administration cost, achieves the effects of treatment and protection and reduces the administration frequency; moreover, the activity and sterilization effect of the sterilization combination have obvious synergistic effect rather than being simple composition of each component; the invention can decelerate the generation of resistance, ensures the high safety of crops and conforms to the safety requirements of pesticide preparations; and the invention has better control effects on alternaria leaf spot, cladosporium cucumerinum, anthracnose, powdery mildew and leaf spot of vegetables, melons and fruits, fruit trees, flowers and cereal crops.