The invention provides an angelica dahurica coumarin extract, wherein the total content of coumarin is not lower than 44.672mg/g, and the content of imperatorin is not lower than 68.623mg/g. The invention further provides an extraction method and a quality control method of the extract. The angelica dahurica coumarin extract has high extraction purity, high content of coumatin, stable quality andfunction of 5-hydroxytryptamine regulation, is capable of treating diseases caused by 5-hydroxytryptamine besides migraine, has distinguished efficacy and strong pertinency of indications, and provides a new choice for clinical treatment.