The invention relates to a method for preparing a virus detection kit in the field of biological systems, which consists of a tissue buffer solution and an amplification system. The method is characterized in that the amplification system is prepared by partially or entirely pre-mixing heat-resistant DNA polymerase, deoxynucleotide triphosphate, a reaction buffer solution and one or more primers for amplification, and is subpackaged for standby; infested water or a tissue extraction solution is directly added into the amplification system, the mixture is mixed evenly, and then the amplification detection can be immediately performed; and particularly when a sample is RNA viruses, the extraction process of nucleic acid is canceled, which can reduce operation steps, can also improve experimental efficiency, and reduce false negative results. The method is suitable for routine examination and emergency detection of medical treatment and other inspection and quarantine fields.