The invention relates to the field of molecular biology and medicine, in particular to a method for screening enhanced anti-tumor compounds by utilizing two channels. Increase in tumor diseases is now number one killer in the world and seriously threatens human survival and health. The prior anti-tumor active compounds screened by aiming at a singe target have the problems of bad patent medicine property or great side effects and the like, and in contrast, medicaments screened through two targets can simultaneously control two transduction pathways. For certain diseases complex in causes, pathological mechanisms and disease development process, multi-target inhibitors are superior to single-target inhibitors in treatment, and multi-target combined blocking-signal transduction is a novel development direction of tumor therapy and medicament development.