The invention relates to a detection method of lead content in gasoline, comprising the following steps: preparing solution, preparing lead standard solution, preparing sample to be detected, drawingstandard curve and detecting the lead content in the gasoline. Compared with GB/T8020, the invention adopts glacial acetic acid-methyl isobutyl ketone solution with mass concentration of 3.0%, improves the stability of a dilution dissolvent system, reduces preparation times of the standard solution, saves resources and avoids environment pollution and hazards to personnel; no lead-poor gasoline isadded in the preparation of the standard solution to reduce the detection limit of the method to 0.02mg/L (0.0428), thus satisfying the need of actual analysis and detection and ensuring the accuracyof data. The detection method used for detecting the same test sample has the advantages of smaller relative standard deviation, high sensitivity and longer stable storage time of work standard solution which can still meet analysis requirement even after being stored for 30 days.