The invention discloses an additive of dyeing process for reclaimed water reusing, comprising the following components by weight percent: 0.5-5% of Bola cationic surface active agent, 10-30% of poly-alpha-hydroxylacrylic acid sodium salt, 5-15% of hydroxycarboxylic acid chelating agent, 2-10% of polyethylene glycol and the balance water. The invention adopts Bola cationic surface active agent with a new structure as microbicide, the microbicide contains two quaternary ammonium salt groups of quaternary ammonium salt so as to increase the insecticide effect effectively and the design of hydrophobic group increases the compatibility to anionic component. At the same time, the surfactant is nonoxidizing biocide and has better storage stability. Aiming to the existing problems in reclaimed water reusing, the formula of the additive adopts one additive to solve different problems caused by the existence of microorganism and heavy metal ions in reclaimed water, thus effectively reducing theuse kind of the additive of dyeing process.