The invention discloses a traditional Chinese medicine composition for treating infertility and a preparation method thereof. The composition comprises the components of dodders, barrenwort, medlar, c. deserticola, codonopsis pilosula, yam, tuckahoe, antlers, raspberries, rhizoma cyperi, radix bupleuri, radix paeoniae alba, angelica, cortex moutan radicis, prepared rehmannia root, and liquorice. The traditional Chinese medicine composition of the invention has reasonable proportion, and any one clinically oral agent or external-use agent can be prepared according to a conventional preparationmethod of traditional Chinese medicine. The composition, in particular matching with meridians buried line therapy, via multiple stimuli effect of acupuncture, medical gut can generate lasting benignstimulation to cutem in process of gradually liquidated absorption with the gut being buried to meridians as foreign proteins so that effect of cutem is more obvious and the composition has exact effect for treating infertility caused by man and woman endocrine disorder, yellow body dysfunction, anovulation, aspermia,atheno-spermia and the like.