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发明授权
CN101659953B 抗猪圆环病毒病shRNA及其设计合成法和用途 失效 - 权利终止

抗猪圆环病毒病shRNA及其设计合成法和用途
摘要：
本发明公开了一种设计合成抗猪圆环病毒shRNA的方法，包括以下步骤：1)、根据PCV-2型病毒的基因组序列，以Rep基因或Cap基因为目的基因，选取一段19bp长的核酸序列；2)、选取同时满足以下3个条件的核酸序列为候选核酸序列：该核酸序列的GC含量为40％-60％，在15-19的位点中至少有3个A或T核酸残基，检查该19bp核酸序列，保证其不会形成二级结构；3)、将所得的候选核酸序列与猪圆环病毒基因组进行比对；以确定该候选核酸序列是特异性的只针对目的基因。本发明的抗猪圆环病毒shRNA，通过抑制猪圆环病病毒在细胞内的复制，从而发挥抗病毒作用。
摘要（英）：

The invention discloses a method for designing and synthesizing anti-PCV shRNA. The method comprises the steps of: 1) selecting a section of nucleotide sequence with length of 19bp according to genome sequence of PCV-2 and by taking Rep gene or Cap gene as target gene; 2) selecting a nucleotide sequence as a candidate nucleotide sequence, which meets the following three conditions: GC content of the nucleotide sequence is between 40 percent and 60 percent; at least three A or T nucleic-acid residues exist among locus from 15 to 10; and secondary structure is ensured not to be generated by 19bp nucleotide sequence after inspection; and 3) comparing the obtained candidate nucleotide sequence with PCV genome so as to determine whether the candidate nucleotide sequence only specially aims at the target gene. The anti-PCV shRNA provided by the invention can play the anti-virus role by restricting reproduction of PCV in cells.

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