The invention discloses a method for testing a non-contact IC card radio frequency protocol, comprising screening interoperability key parameter, constructing a testing system, calibrating the testing system and testing, wherein screening is carried out according to the influence of the parameters to the interoperability, and the key parameter influencing the interoperability of a non-contact IC card can be found out in the parameters, so that the testing method is the only way for solving the problem of interoperability; and the testing system comprises professional testing software, a transceiver specially used for testing, an ISO 10373-6 test suite, a power regulating suite, a monitoring and protocol analyzing device, an oscilloscope and a connecting line used for testing.The invention has the advantages that by adopting multi-loop synchronous verification technique, working mode which can be used for completing every test scene based on script and modular software layered design technology, the invention effectively ensures the stability of the testing system as well as the authenticity and the recurrence of the test result, leads the test content to be updated and the problem analysis to be simple and flexible, and guarantees the function of the testing software to be expandable and the maintenance to be convenient and easy.