The invention relates to a negative-pressure in-vitro cell culture apparatus which solves the problems that the existing cell proliferation is slow and is difficult to meet the requirement of tissue engineering cell and provide a device of an ideal liquid-gas interface for cell culture. The negative-pressure cell culture apparatus comprises a liquid storage tank, a silicone tube, a glass tube, a diaphragm capsule manometer, a flow valve, a high-pressure gas cylinder, a mini vacuum pump, a peristaltic pump, a micropore filter membrane and a filter of 0.22 Mu, wherein the liquid storage tank is connected with a cell culturing vat by the glass tube; and the cell culturing vat is connected by the glass tube provided with the diaphragm capsule manometer and the flow valve, and forms negative pressure by the mini vacuum pump. The cell is connected with the liquid storage tank by the silicone tube provided with the peristaltic pump; and the high-pressure gas cylinder is connected with the liquid storage tank by the silicone tube, thus not only being capable of guaranteeing the environment of carbon dioxide required by cell growth, but also being capable of forming negative pressure. The negative-pressure in-vitro cell culture apparatus not only can accurately imitate the pressure environment of in-vivo cells and provide better support for in-vivo experiment, but also can amplify celltypes in great amount in vitro.