The invention discloses a dyeing and finishing process of corn fibers and cotton blending and mixed fabrics, comprising the following steps: end stitching of greige cloth, pre-setting, pre-treatment, dyeing, dehydration, post finishing, and warehouse entry inspection. In the pre-setting process, the temperature is 120-130 DEG C and the time is 30-45 minutes; in the pre-setting process, the presetfabrics are placed in an overflow dying machine to conduct desizing and remove the hairiness on the surface of the fabrics; in the dying process, the corn fibers adopt middle temperature type and lowtemperature type dispersing dyes to conduct dying; and in the post finishing process, the dyed fabrics are placed in a 8-12g/L of hydrophilic amino-silicone softener for padding, and then the padded fabrics are dried and stentered and set at 100-120 DEG C. The dyeing and finishing process of corn fibers and cotton blending and mixed fabrics has good coloring rate, ensures the strength and feel ofcorn fibers, and also has significant impact on the industrial development of the textile industry.