The present invention provides a camera lens calibration method and system for improving calibration efficiency and saving human resource. The method comprises the following steps: acquiring the position information corresponding to the specified detected area of a die to be detected; utilizing a Camera to be detected to detect an element to be detected; obtaining a practical detected area and corresponding position information and calibration characteristic parameter information; obtaining adjusting parameter information; and adjusting the practical detected area according to the position information, the calibration characteristic parameter information and the adjusting parameter information to enable the practical detected area to coincide with the specified detected area.