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CN101692074A 一种液相色谱法分析脂肪酰胺类化合物的方法 无效 - 驳回

一种液相色谱法分析脂肪酰胺类化合物的方法
摘要：
一种液相色谱法分析测定长链[C8～24]脂肪酰胺类化合物的方法，其特征在于：以高纯球型硅胶微粒，反相键合C18为填料的色谱柱，以水—有机极性溶剂为流动相，其中流动相中包括有机酸；水—有机极性溶剂体积比为80∶20～50∶50；包含以下步骤：(1)取长链[C8～24]脂肪酰胺类化合物样品适量，用水溶解样品，并配置成每1ml含长链[C8～24]脂肪酰胺类化合物0.1～5mg的样品溶液；(2)设置流动相的流速为0.5～1.5ml/min，检测波长为200～260nm，色谱柱柱箱温度为25～50℃；(3)取(1)的样品溶液10～40μl，注入高效液相色谱仪，完成长链[C8～24]脂肪酰胺类化合物的分析。
摘要（英）：

The invention provides a method for determining long-chain [C8-C24] fatty amide compounds on the basis of liquid chromatography (LC). The method is characterized in that high-purity spherical silica gel particles are reverse-bonded with chromatographic column with C18 as filler; and water-organic polar solvent with the volume ratio being 80:20-50:50 is taken as a mobile phase containing organic acid. The method comprises the following steps: (1) sampling a proper amount of long-chain [C8-C24] fatty amide compounds, and dissolving the sample in water, to prepare 0.1-5mg of sample solution out of 1ml of the long-chain [C8-C24] fatty amide compounds; (2) setting the flow rate of the mobile phase as 0.5-1.5ml/min, the detection wavelength as 200-260nm and the temperature of the chromatographic column box as 25-50 DEG C; and (3) injecting 10-40 mu l of the sample solution in Step (1) into the high-performance liquid chromatograph, so as to finish the analysis of long-chain [C8-C24] fatty amide compounds.

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