The invention discloses an ambulation training device for spinal cord injury rats, which comprises an ambulation device, a weight loss device and a power assisting device, wherein the power assisting device includes fixing supports arranged on two sides of the ambulation device. Rotary rods are mounted on each fixing support and above the ambulation device; an upper rotary wheel is arranged on each rotary rod; a lower rotary wheel driven to rotate by the movement of the ambulation device is disposed below the upper rotary wheel; the upper rotary wheel and the lower rotary wheel are connected through transmission belts; a push rod is disposed between each rotary rod and the ambulation device and connected with the rotary rod; a power transmission rod is arranged right ahead the push rod; a fixing rod for suspending the power transmission rod is installed on each fixing support; the rotary rod transmits power to the power transmission rod via the push rod; the power transmission rod is connected with posterior limbs of an animal; and the weight loss device is fixed with the animal. The ambulation training device can simultaneously conduct active training and power assisting training for animals with different degrees of spinal cord injury, and solve the problem of synchronous movement training for a plurality of spinal cord injury animals.