The invention relates to a preparation method of macroporous absorption resin, which comprises the following steps of adding framework raw materials, a pore-forming agent, an initiating agent and a dispersing agent into deionized water, fully stirring and mixing; sequentially raising the temperature of a liquid mixture for suspension polymerization by four stages to gradually cure the liquid mixture to oyster white 20-60mu spherical particles, wherein the framework raw materials are divinylbenzene with the weight percentage concentration of 80 percent, the pore-forming agent is one of isooctane and n-pentane, the initializing agent is benzoyl peroxide, and the dispersing agent is polyvinyl alcohol; transferring the oyster white spherical particles into an elution column, introducing vapour with temperature of 130-150 DEG C as an eluting agent, eluting the pore-forming agent remained in the oyster white spherical particles; and sieving the obtained oyster white spherical particles to obtain the 20-60mu macroporous absorption resin with specific surface area more than 800m2/g. The method has little pollution and low production cost; and the prepared macroporous absorption resin has better property.