The invention discloses a starter culture for liquor-making with uncooked materials, relating to additive in the field of food processing. The starter culture is prepared from the following steps: completely and evenly stirring heat-resistant brewing active yeast, aspergillus koji, high transformed diastatic enzyme, alpha-amylase, aroma-producing yeast, Wuyi red distiller's yeast, bran yeast and pectinase; crushing at below 25 DEG C; putting into a stirrer to stir for 15-20 minutes; and sifting through a 70 mesh sifter to obtain the starter culture. The invention has the characteristics of shorter ferment time, smaller usage of the starter culture, higher liquor-acquiring rate, fuller flavor of brewed liquor, better taste and the like, has simple preparation and brewing process and is suitable for amylum-containing various plants for liquor-making.