The invention relates to a deproteinized calf blood extractive injection, every milliliter of which contains 160-200 mg of total solid, 100-150 mg of xylitol and 60-100 mg of epsilon-polylysine, wherein the total solid comprises the following components in parts by weight: 10-20 parts of amino acid, 25-35 parts of polypeptide and 15-25 parts of inorganic salt. The preparation method comprises thefollowing steps: taking calf blood and sodium citrate, adding water and stirring to rise the temperature, quickly cooling to -10-0 DEG C while stirring, centrifugating, regulating the pH value of thesupernatant, adding pepsin for enzymolysis, filtering, regulating the pH value, adding subtilisin for enzymolysis, and filtering; adding ethanol water solution to the filtrate, stirring, centrifugating, letting the supernatant flow through active carbon columns, combining the eluent and the cross-flow solution, concentrating until no ethanol smell is emitted, adding the xylitol and the epsilon-polylysine to the concentrated solution, adding water to dilute, and ultrafiltering; and packaging and filling the ultrafiltrate, sterilizing with ultraviolet light, and packing to obtain the injection.