The invention discloses a bactericidal composition containing cyproconazole and famoxadone. The weight ratio of cyproconazole to famoxadone in the composition is 5:1-1:20, and the composition can be prepared into water dispersible granules, wettable powder, suspension concentrates, dispersible oil miscible flowable concentrates, water emulsion, micro-emulsion or emulsion concentrates. The bactericidal composition can be used for preventing and treating powdery mildew, banana leaf spot disease, apple leaf spot drop, ring rot, citrus scab and jujube rust of melons, grapes and fruit trees. The bactericidal composition has reasonable components, both the prevention and the treatment roles and good bactericidal effect, and can reduce the application times and reduce the application cost; furthermore, the activity and the bactericidal effect of the bactericidal composition are not the simple overlapping of the activities of all the components, and all the components realize the significant synergy, thereby retarding the production of drug resistance, having good safety to crops and being in line with the safety requirement of pesticide preparations.