The invention relates to a preparation method of gravure water-based ink. Most of the existing ink is not environment-friendly enough. The preparation method comprises the following steps: firstly, mixing and evenly stirring acrylic acid, styrene, vinyl acetate, water, an emulsifier and an initiator to obtain acrylic acid-styrene-vinyl acetate copolymer emulsion interpenetrating network-type water-soluble resin; then placing the acrylic acid-styrene-vinyl acetate copolymer emulsion interpenetrating network-type water-soluble resin and pigment, water, isopropyl alcohol, ethanol, a defoamer, a wetting agent, a leveling agent, a dispersant, a coupling agent and an antibacterial agent in an ink preparing barrel, and stirring to form a pasty ink material; and placing the pasty ink material in a three-roller ink grinding mill for ink grinding to lead pigment particles to be smashed completely and uniformly dispersed into a liquid binder, thus preparing the gravure water-based ink. The method is simple in technology, and the prepared ink has good printability, moisture friction resistance, high gloss and good adhesion, therefore, the gravure water-based ink can be used for different types of paper.