The invention discloses a recombinant human herpes simplex virus I protein HSV I gG and application thereof. In the invention, a computer is used for analyzing all amino acid sequences of the human herpes simplex virus I so as to screen out the dominant antigen epitopes of the type-specific proteins gG of the human herpes simplex virus I, and the recombinant human herpes simplex virus I protein HSV I gG, which is produced by the genetic engineering method and simply named as HSV IgG, can avoid the problem that the specificity detection is lowered due to false positive caused by low purity of protein antigens or poor specificity, and provide more specific and accurate raw materials to detect the infection of the human herpes simplex virus. Compared with the similar kits, the diagnosis kit prepared by using the recombinant human herpes simplex virus I protein HSV I gG as the antigen has the advantages of strong specificity, high sensitivity and the like, and better meets the needs of clinically diagnosing the infection of the human herpes simplex virus; and when inoculated as a vaccine, the recombinant human herpes simplex virus I protein HSV I gG can prevent virus infection.