利索-全球专利检索

  1. 登录
  2. 注册
发明授权
CN101705265B 利用工程菌株M18G携带质粒pME6032Phz生产吩嗪-1-羧酸的方法 失效 - 权利终止

  • 专利标题： 利用工程菌株M18G携带质粒pME6032Phz生产吩嗪-1-羧酸的方法
  • 专利标题（英）： Method for producing phenazine-1-carboxylic acid by using engineering bacterial strain M18G to carry plasmid pME6032Phz
  • 申请号： CN200910198664.2
    申请日： 2009-11-12
  • 公开(公告)号： CN101705265B
    公开(公告)日： 2011-07-27
  • 发明人: 许煜泉 , 周泉
  • 申请人： 上海交通大学
  • 申请人地址： 上海市闵行区东川路800号
  • 专利权人： 上海交通大学
  • 当前专利权人： 上海交通大学
  • 当前专利权人地址： 上海市闵行区东川路800号
  • 代理机构： 上海交达专利事务所
  • 代理商 毛翠莹
  • 主分类号： C12N15/63
  • IPC分类号： C12N15/63 ;  C12R1/01
利用工程菌株M18G携带质粒pME6032Phz生产吩嗪-1-羧酸的方法
摘要：
本发明涉及一种利用工程菌株M18G携带质粒pME6032Phz生产吩嗪-1-羧酸的方法，从促生拮抗菌M18基因组中扩增出吩嗪-1-羧酸生物合成的编码基因片段phzA1-G1，并将该基因片段插入表达质粒pME6032中，置于强启动子Ptac的控制下，构建成重组质粒pME6032Phz；然后将该重组质粒导入促生拮抗菌M18的衍生菌株M18G中，构建成基因工程菌株M18G/pME6032Phz。该工程菌株在扩增吩嗪-1-羧酸生物合成编码基因的拷贝数的同时，实现编码基因的高效稳定表达。最后将基因工程菌株M18G/pME6032Phz在黄豆粉培养液中培养，高效稳定地生产吩嗪-1-羧酸，大幅提高吩嗪-1-羧酸的产量。利用本发明的高产基因工程菌株制备吩嗪-1-羧酸，其生产成本能达到应用于防治水稻纹枯病的农本要求，可用于杀菌剂申嗪霉素的制备，大规模地防治植物病害。
摘要（英）：

The invention relates to a method for producing phenazine-1-carboxylic acid by using engineering bacterial strain M18G to carry plasmid pME6032Phz. The method is implemented by amplifying coding gene segment phzA1-G1 from cerebiogen antagonist antibiosis M18 genome for biosynthesis of phenazine-1-carboxylic acid, inserting the gene segment into expression plasmid pME6032, placing under control ofstrong promoten Ptac to construct a recombinant plasmid pME6032Phz; then introducing the recombinant plasmid in the derivative bacterial strain M18G of cerebiogen antagonist antibiosis M18 to construct an engineering bacterial strain M18G/pME6032Phz. The engineering bacterial strain can realize efficient and stable expression of coding genes while amplifying reproduction number of coding genes for biosynthesis of phenazine-1-carboxylic acid. Finally the engineering bacterial strain M18G/pME6032Phz is cultured in soybean meal culture solution to efficiently and stably produce phenazine-1-carboxylic acid with vastly enhanced yield. By using the high yield engineering bacterial strain to produce phenazine-1-carboxylic acid, the production cost can meet the agricultural requirement for application in preventing and curing rice sheath blight disease, and the invention is applicable to preparation of bactericide phenazino-1-carboxylic acid and can be used for preventing and curing plant diseases in large scale.

公开/授权文献
0/0