Provided is a method of producing a multilayer coating film, including the steps of: turning multiple coating liquids into multiple layers in advance; and transferring the coating liquids turned into multiple layers onto a substrate, in which a coating liquid having the following characteristics is inserted as an intermediate layer between two kinds of coating liquids to be laminated so that the coating liquids are turned into multiple layers: the coating liquid contains both film-forming components in the two kinds of coating liquids, and a total concentration of the film-forming components is higher than a concentration of the film-forming component in each of the two kinds of coating liquids. The production method includes producing a multilayer coating film extremely excellent in interlayer adhesiveness with ease and good productivity, by collectively applying multiple coating liquids without using a gelling agent or the like for modifying the viscosity of each coating liquid to be laminated.