The invention relates to the chemical technical field of mesoporous material preparation and analysis, in particular to a hybrid ordered mesoporous silica gel chromatograph stationary-phase stuffing and a preparation method thereof. The hybrid ordered mesoporous silica gel chromatograph stationary-phase stuffing is characterized in that: hydroxyl connected with silicon atoms on the original hybrid ordered mesoporous silica gel is substituted by aminopropyl. The stuffing has regular and smooth spherical appearance; frameworks of the silicon gel are doped with ethylene radical which has weak hydrophobicity, therefore, the stuffing can not generate silicon gel dissolution phenomenon when being used in flow phase with high aqueous phase proportion and can bear larger pH range (1-11); furthermore, mesoporous pore paths are distributed sequentially, therefore, the mass transfer can be accelerated, and the column pressure is reduced; and the invention is suitable for fast segregation analysis under high aqueous phase proportion.