The invention belongs to the technical field of tissue engineering and gene delivery, in particular to a double-gene activated bone-cartilage compound transplant, a preparation method and application thereof. In the method, seed cells are respectively planted on two layers of bracket materials which are activated by different genes and have the same main constituents, and the seed cells of the upper layer and the lower layer are simultaneously induced to respectively differentiate to chondrocyte and osteoblast by utilizing an in situ gene transfection principle so as to form a tissue engineering bone-cartilage compound transplant in vitro, wherein the seed cells are bone mesenchymal stem cells; the main constituents of the bracket materials are chitosan and gelatin; and in the two different genes, one is a gene differentiated from any known induced stem cell to the chondrocyte, while the other one is a gene differentiated from any known induced stem cell to the osteoblast. By utilizing the method, the bone-cartilage compound transplant is prepared in vitro and further used for restoring bone-cartilage.