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发明公开
CN101724573A 一种高效表达几丁质酶编码基因和β-1,3-葡聚糖酶编码基因的木霉生防重组工程菌及其应用 无效 - 撤回

一种高效表达几丁质酶编码基因和β-1,3-葡聚糖酶编码基因的木霉生防重组工程菌及其应用
摘要：
本发明公开了一种可高效表达木霉来源几丁质酶和β-1，3-葡聚糖酶基因的木霉重组工程菌及其应用。本发明的重组菌株是通过土壤杆菌介导的含木霉来源几丁质酶编码基因chi42和β-1，3-葡聚糖酶编码基因glultr-2的T-DNA衍生载体转化具有植物病害生物防治功能的绿色木霉LTR-2获得的重组工程菌。重组菌可以在PDA培养基稳定表达几丁质酶编码基因chi42和β-1，3-葡聚糖酶编码基因glultr-2，盆栽实验表明重组工程菌对蔬菜灰霉病和小麦纹枯病具有比出发菌LTR-2，更有效的防治效果。
摘要（英）：

The invention discloses trichoderma biocontrol recombinant engineering bacteria for efficiently expressing chitinase and Beta-1,3-glucanase genes derived from trichoderma as well as application thereof. The recombinant bacterial strain is the recombinant engineering bacteria which are acquired by converting trichoderma viride LTR-2 with plant disease biocontrol function from a T-DNA derived carrier which is mediated by agrobacterium and contains the chitinase code gene chi42 derived from the trichoderma and the Beta-1,3-glucanase coding gene glultr-2. The recombinant bacteria can stably express the chitinase code gene chi42 and the Beta-1,3-glucanase coding gene glultr-2 in a PDA culture medium; and a pot plant experiment indicates that the recombinant engineering bacteria have more effective prevention and control effects to vegetable botrytis and wheat sharp eyespot compared with glutamicum LTR-2.

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